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mouse gas6 duoset elisa kit (R&D Systems)

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R&D Systems mouse gas6 duoset elisa kit

AXL is highly expressed on ID8-derived cell lines. A. Stacked histogram plot showing expression of AXL on different passages of the ID8 cell lines compared to the FMO. B. Barplot showing the change in median fluorescence intensity of AXL (dMFI) in the different ID8 cell lines. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. C – H. Immunofluorescence images of the different ID8 cell lines at 40X magnification. Blue, DAPI; Red, AXL. I. Barplot showing the kinetic changes in the concentration of serum <t>GAS6</t> (pg/mL) of P3 ID8 Thy1.1 tumor-bearing mice across weeks 1–6 post-inoculation and age-matched naïve mice. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. *, p < 0.05.

Mouse Gas6 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 13 article reviews

mouse gas6 duoset elisa kit - by Bioz Stars, 2026-04

93/100 stars

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1) Product Images from "A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib"

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

Journal: Gynecologic Oncology Reports

doi: 10.1016/j.gore.2025.101998

Figure Legend Snippet: AXL is highly expressed on ID8-derived cell lines. A. Stacked histogram plot showing expression of AXL on different passages of the ID8 cell lines compared to the FMO. B. Barplot showing the change in median fluorescence intensity of AXL (dMFI) in the different ID8 cell lines. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. C – H. Immunofluorescence images of the different ID8 cell lines at 40X magnification. Blue, DAPI; Red, AXL. I. Barplot showing the kinetic changes in the concentration of serum GAS6 (pg/mL) of P3 ID8 Thy1.1 tumor-bearing mice across weeks 1–6 post-inoculation and age-matched naïve mice. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. *, p < 0.05.

Techniques Used: Derivative Assay, Expressing, Fluorescence, Immunofluorescence, Concentration Assay

High expression of AXL and GAS6 mRNA in OC patients correlates with worse outcome. A-D. Kaplan-Meier plots showing the hazard ratio (HR) and logrank P value in OC patients for A. AXL mRNA for overall survival (OS) ( AXL- high: n = 169, AXL- low: n = 204); B. GAS6 mRNA for OS ( GAS6- high: n = 125, GAS6- low: n = 248); C. GAS6 mRNA for recurrence-free survival (RFS) ( GAS6- high: n = 122, GAS6- low: n = 55); D. mean expression of AXL and GAS6 mRNA for OS ( AXL and GAS6 -high: n = 203, AXL and GAS6- low: n = 170). E. Heatmap showing high AXL or GAS6 mRNA expression in different tissues from OC patients. F. Heatmap showing high AXL or GAS6 mRNA expression in platinum-resistant or sensitive OC patients.

Figure Legend Snippet: High expression of AXL and GAS6 mRNA in OC patients correlates with worse outcome. A-D. Kaplan-Meier plots showing the hazard ratio (HR) and logrank P value in OC patients for A. AXL mRNA for overall survival (OS) ( AXL- high: n = 169, AXL- low: n = 204); B. GAS6 mRNA for OS ( GAS6- high: n = 125, GAS6- low: n = 248); C. GAS6 mRNA for recurrence-free survival (RFS) ( GAS6- high: n = 122, GAS6- low: n = 55); D. mean expression of AXL and GAS6 mRNA for OS ( AXL and GAS6 -high: n = 203, AXL and GAS6- low: n = 170). E. Heatmap showing high AXL or GAS6 mRNA expression in different tissues from OC patients. F. Heatmap showing high AXL or GAS6 mRNA expression in platinum-resistant or sensitive OC patients.

Techniques Used: Expressing

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Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: AXL is highly expressed on ID8-derived cell lines. A. Stacked histogram plot showing expression of AXL on different passages of the ID8 cell lines compared to the FMO. B. Barplot showing the change in median fluorescence intensity of AXL (dMFI) in the different ID8 cell lines. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. C – H. Immunofluorescence images of the different ID8 cell lines at 40X magnification. Blue, DAPI; Red, AXL. I. Barplot showing the kinetic changes in the concentration of serum GAS6 (pg/mL) of P3 ID8 Thy1.1 tumor-bearing mice across weeks 1–6 post-inoculation and age-matched naïve mice. Error bars indicate SEM. Statistical significance was determined using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. *, p < 0.05.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Derivative Assay, Expressing, Fluorescence, Immunofluorescence, Concentration Assay

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Figure Lengend Snippet: High expression of AXL and GAS6 mRNA in OC patients correlates with worse outcome. A-D. Kaplan-Meier plots showing the hazard ratio (HR) and logrank P value in OC patients for A. AXL mRNA for overall survival (OS) ( AXL- high: n = 169, AXL- low: n = 204); B. GAS6 mRNA for OS ( GAS6- high: n = 125, GAS6- low: n = 248); C. GAS6 mRNA for recurrence-free survival (RFS) ( GAS6- high: n = 122, GAS6- low: n = 55); D. mean expression of AXL and GAS6 mRNA for OS ( AXL and GAS6 -high: n = 203, AXL and GAS6- low: n = 170). E. Heatmap showing high AXL or GAS6 mRNA expression in different tissues from OC patients. F. Heatmap showing high AXL or GAS6 mRNA expression in platinum-resistant or sensitive OC patients.

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Expressing

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Derivative Assay, Expressing, Fluorescence, Immunofluorescence, Concentration Assay

Journal: Gynecologic Oncology Reports

Article Title: A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib

doi: 10.1016/j.gore.2025.101998

Article Snippet: Quantification of the serum concentration of GAS6 was performed using the Mouse GAS6 DuoSet ELISA kit (R&D systems, cat# DY986) according to manufacturer instructions.

Techniques: Expressing

(A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

(A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Staining, Derivative Assay, Cell Culture, Recombinant

(A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Gene Expression, Derivative Assay, Cell Culture, Recombinant, Concentration Assay

(A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Transgenic Assay, Concentration Assay, Staining, Derivative Assay

(A) Prognostic value of GAS6 expression in acute myeloid leukemia (TCGA LAML, n=173). Data was generated using the KaplanScan mode from the R2 Genomics Analysis and Visualization Platform (http://r2.amc.nl). Survival analysis by log-rank (Mantel–Cox) test.

Journal: Cancer discovery

Article Title: AXL inhibition in macrophages stimulates host- versus -leukemia immunity and eradicates naive and treatment resistant leukemia

doi: 10.1158/2159-8290.CD-20-1378

Figure Lengend Snippet: (A) Prognostic value of GAS6 expression in acute myeloid leukemia (TCGA LAML, n=173). Data was generated using the KaplanScan mode from the R2 Genomics Analysis and Visualization Platform (http://r2.amc.nl). Survival analysis by log-rank (Mantel–Cox) test.

Article Snippet: Samples were then analyzed using the Mouse Gas6 DuoSet ELISA (DY986: R&D Systems), according to the manufacturer’s instructions.

Techniques: Expressing, Generated

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1) Product Images from "A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and Olaparib"

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